SMARTer_RACE_5_3__Kit_User_Manual_022714(6)

clontech试剂盒 SMARTer_RACE 5'/3' Kit User Manual

Figure 2. Overview of the SMARTer RACE procedure. Detailed flow charts of the SMARTer RACE mechanisms can be found in

Appendices B & C. Alternatively, you can obtain the sequences of the extreme ends of the transcript by sequencing the 5’ end of the 5’ product and the 3’ end of the 3’ product. Using this information, you can design 5’ and 3’ gene-specific primers to use in LD PCR with the 5’-RACE-Ready cDNA as template to generate the full-length cDNA. Note that with the cloned RACE fragments you can use a restriction site in an overlapping region to construct a full-length cDNA by subcloning, or design new GSPs to generate PCR products compatible with In-Fusion cloning.

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