SMARTer_RACE_5_3__Kit_User_Manual_022714(3)

clontech试剂盒 SMARTer_RACE 5'/3' Kit User Manual

Figure 1. Mechanism of SMARTer cDNA synthesis ............................................................................................................. 4

Figure 2. Overview of the SMARTer RACE procedure.. ....................................................................................................... 6

Figure 3. The relationship of gene-specific primers to the cDNA template. .......................................................................... 9

Figure 4. 5'- and 3'-RACE sample results ............................................................................................................................. 22

Figure 5. Detailed mechanism of the 5'-RACE reactions. .................................................................................................... 27

Figure 6. Detailed mechanism of the 3'-RACE reactions. .................................................................................................... 28 Table of Tables

Table 1. Additional 5'-RACE Sequence Obtained with SMART Technology ....................................................................... 5

Table 2. Setting up 5'- and 3'-RACE PCR Reactions ........................................................................................................... 15

Table 3. Troubleshooting Guide: Other Specific Problems .................................................................................................. 25

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