常用培养基配制(6)

参考文献

1. Atlas, R.M., Handbook of Microbiological Media, second edition, Ed. Parks,

L.C., CRC Press, N.Y., 1997.

2. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, second edition,

Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, A1.2-A2.12, 2001.

常用缓冲液

10X 磷酸盐缓冲液(储存液) (PBS) NaCl KCl Na2HPO4 KH2PO4 H2O HCl H2O 20X SSC NaCl

Na3citrate x H2O H2O

1M HCl调节pH值至7.0

/升 80.0 g 2.0 g 14.4 g 2.7 g 至800 ml 至pH 7.4 至1升 /升 175.3 g 88.2 g 至800 ml

终1 X浓度 137 mM 2.7 mM 100 mM 2 mM

终1 X浓度 150 mM 15 mM

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