大孔吸附树脂分离纯化迷迭香中迷迭香酸的研究

【摘要】  目的考察大孔吸附树脂对迷迭香酸的吸附性能和纯化效果，寻找从迷迭香中分离纯化迷迭香酸的较优工艺。方法以迷迭香酸的吸附量、解吸率和所得粉末中迷迭香酸的含量为指标，从4种大孔吸附树脂中筛选出较好的D101树脂。通过静态和动态实验，对迷迭香酸在D101树脂上吸附和解吸的条件进行考察和优化。结果采用D101树脂从迷迭香中分离纯化迷迭香酸的较优工艺参数为：上柱液pH 3～4，速度2 BV/h，溶液处理量9 BV，洗脱剂为40%乙醇，洗脱速度1 BV/h，收集洗脱液3 BV。按此工艺条件，迷迭香酸的解吸率为94.8%，3 BV洗脱液浓缩干燥后，所得粉末中迷迭香酸含量为23.7%，树脂经8次重复使用，吸附量和分离效果无明显下降，可以重复使用。结论 D101树脂可从迷迭香中较好的分离纯化迷迭香酸，具有潜在的工业应用价值。

【关键词】  迷迭香酸； 大孔吸附树脂； 迷迭香； 分离； 纯化

    ChinaAbstract:ObjectiveTo evaluate the absorption capability and purification effect of rosmarinic acid on macroporous absorption resin, and to find a better method to separate and purify rosmarinic acid from Rosmarinus officinalis L.. MethodsMacroporous resin D101 was more suitable for the separation and purification of rosmarinic acid among four kinds of resins selected with absorption capacity, desorption ratio and the content of rosmarinic acid in the powder obtained as criteria．Optimal conditions for absorption and elution of rosmarinic acid were performed with static and dynamic experiments.ResultsThe result showed that the optimum technology parameters were as follows：the pH value of solution 3 to 4, flow rate 2 BV/h, solution treatment capacity 9 BV, and rosmarinic acid absorbed can be desorbed by 40% alcohol at flow rate 1 BV/h, and collected 3 BV eluent. The desorption ratio of rosmarinic acid was 94.8% according to the absorption and desorption conditions above, and the content of rosmarinic acid in powder obtained from 3 BV eluent through concentration and dry was 23.7%. After eight times of repeated use, the absorption capability and separation effect of the macroporous resin was not decreased obviously, and the macroporous resin could be recycled.ConclusionRosmarinic acid in Rosmarinus officinalis L. can be effectively separated and purified with the macroporous absorption resin D101, and has the potential application value in industry.

　　Key words:Rosmarinic acid；Macroporous absorption resin；  Rosmarinus officinalis L.；  Separation；  Purification
   
　　迷迭香Rosmarinus officinalis L.系唇形科迷迭香属植物，为多年生常绿小灌木，原产于地中海地区，国内近年来大面积的引种栽培。该植物具有抗氧化、抗病毒和抗肿瘤等活性，广泛应用于医药、食品和饮料等行业。研究发现［1～4］：该植物所含的水溶性酚酸成分—迷迭香酸（Rosmarinic acid）具有明显的抗氧化、抗炎、免疫抑制、抗血栓、抗菌、抗病毒等生物活性。
   
　　迷迭香酸不仅迷迭香植物含有，也存在于其他唇形科植物如紫苏、丹参，但近年来国外市场对从迷迭香中提取的不同含量的迷迭香酸粗品比从其他植物中提取更为关注。关于从迷迭香中提取迷迭香酸的研究有文献报道［5］，但未见提取后纯化工艺的研究，更未见采用大孔吸附树脂分离纯化迷迭香中迷迭香酸的报道。

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