常用pGEX载体图谱(7)

pET23a载体简介

The pET-23a-d(+) vectors carry an N-terminal T7?Tag? sequence plus an optional C-terminal His?Tag? sequence. These vectors differ from pET-21a-d(+) by the “plain” T7 promoter instead of the T7lac promoter and by the absence of the lacI gene.

Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

pGEXKG载体基本信息

出品公司:

别名: 质粒类型: 表达水平: 启动子: 克隆方法: 载体大小:

5' 测序引物及序列: 3' 测序引物及序列:

载体标签: 载体抗性: 备注: 产品目录号: 稳定性: 组成型: 病毒/非病毒:

ATCC

pGEX-KG, pGEXKG, pGEX KG 大肠杆菌蛋白表达载体 高拷贝 Tac

多克隆位点，限制性内切酶, BamHI，SmaI, EcoRI, XbaI, NcoI, SalI, XhoI, SacI, HindIII 5006 bp

pGEX5': GGGCTGGCAAGCCACGTTTGGTG pGEX3': CCGGGAGCTGCATGTGTCAGAGG N-GST

Ampicillin 氨苄

复制子是pMB1, 来源于pGEX-2T 77103

瞬时表达 Transient 诱导表达 非病毒

pGEXKG载体质粒图谱和多克隆位点信息

pMAL-C2X 表达载体使用直链淀粉树脂（amylose resin）分离融合蛋白

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