双氯芬酸钾聚乳酸静电纺丝膜的制备 - 图文

题目：毕业论文

聚乳酸电纺材料载药性能的研究 学 生： 刘 俐 学 号： 201004030210 院 （系）： 生命科学与工程学院 专 业： 药物制剂 指导教师： 王 兰 沈 文

2014 年6月6日

I

聚乳酸电纺材料载药性能的研究

摘 要

静电纺丝是高分子流体静电雾化的特殊形式，聚合物微小射流在高压电场下运行一定距离后固化成纤维。通过静电纺丝技术制备载药释放纳米纤维支架是近年来世界医学科学技术领域重要课题。静电纺丝以其制造装置简单、纺丝成本低廉、可纺物质种类繁多、工艺可控等优点，已成为有效制备纳米纤维材料的主要途径之一。

本课题将模型药物双氯芬酸钾和聚乳酸同时溶解在三氯甲烷/乙醇（体积比75/25）的二元溶剂中，通过扫描电镜表征优化电场条件，并用静电纺丝技术制备载药PLA/双氯芬酸钾复合纳米纤维薄膜。借助扫描电镜观察纤维形貌，用溶出仪及紫外分光光度计检测其释放在人工汗液中药物的吸光度，并计算其释药速率，建立释放模型。

本研究结果表明：聚乳酸和双氯芬酸钾能很好地形成载药体；双氯芬酸钾质量分数为2%时复合纤维释药速率最大；药物释放速率随着药物质量分数的增大而增加，释放稳定性随药物物质量分数的增大而减小。药物释放参考Higuchi模型拟合，发现拟合度较好，可以预测某一时刻药物的释放量。 关键词 聚乳酸，静电纺丝，释放模型

II

The Research of Medicine Performance of the Poly-lactic Acid

Electro-spun Material

ABSTRACT

Electro spinning is a special form of the poly fluid electromagnetic atomizing,the polymer micro-jets run at a considerable distance in a high voltage field and turn into solidified fibers.Nano fiber material prepared by electro spinning is the most important academic and technical activities over the last decade in the world of material science and technology.Electro spinning has become one of the main ways in the effective preparation of nano fiber material because of its simple manufacturing,low cost ,a wide variety of materials spinning,process controllable,etc. Diclofenac

potassium

and

poly-lactic

acid

will

be

dissolved

in

chloroform/ethanol(volume ratio 75/25)binary solvent simultaneously.Electric field conditions can be optimized by scanning electron microscopy to gain the characterization of nano fiber film and preparing drug-loaded PLA/diclofenac potassium composite nano fiber film by electro-spinning technique.Fiber morphology can be seen by scanning electron microscopy and absorbance of drug release in artificial sweat with the dissolution tester and UV spectrophotometer,then calculate its release rate and build the release model.

The results showed that:PLA and diclofenac potassium drug can be well formed;the drug release rate of the composite fiber is maximum when the diclofenac potassium mass fraction is 2%;release rate of the drug increases with the mass fraction,the release stability of the drug increases with lower mass fraction.Higuchi reference drug release model fitting is good. KEY WORDS Poly-lactic acid,electro spinning,release model

III 目 录

摘 要 .................................................................... I ABSTRACT ................................................................ II 1.绪论 ................................................................... 1

1.1生物高分子材料 .................................................... 1

1.1.1生物高分子材料的分类 ......................................... 1 1.1.2生物高分子材料的应用 ......................................... 2 1.2聚乳酸及其载药系统 ................................................ 3

1.2.1聚乳酸及其应用 ............................................... 3 1.2.2聚乳酸载药系统的研究进展 ..................................... 5 1.3静电纺丝 .......................................................... 6

1.3.1静电纺丝技术原理 ............................................. 7 1.3.2静电纺丝纤维的形态及影响因素 ................................. 7 1.3.3静电纺丝技术的应用 ........................................... 8 1.3.4聚乳酸电纺及其应用进展 ...................................... 10 1.4静电纺丝载药与药物释放 ........................................... 12

1.4.1静电纺丝载药的研究现状 ...................................... 12 1.4.2药物释放系统 ................................................ 13 1.4.3药物释放的评价方法 .......................................... 15 1.5课题研究的意义及创新 ............................................. 18

1.5.1课题研究意义 ................................................ 18 1.5.2创新性 ...................................................... 18

2.实验部分 .............................................................. 19

2.1原料和设备 ....................................................... 19

2.1.1实验原料 .................................................... 19 2.2.2实验仪器 .................................................... 19 2.2实验方法 ......................................................... 19

2.2.1溶剂体系的选择 .............................................. 19 2.2.2 PLA溶液的配制 .............................................. 19 2.2.3静电纺丝纳米纤维膜的制备 .................................... 20 2.2.4 PLA/双氯芬酸钾混合溶液的配制及纳米纤维膜的制备 ............. 20 2.2.5纳米纤维膜的性能表征 ........................................ 20

3.结果与讨论 ............................................................ 22

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